Protein purification Study guides, Class notes & Summaries

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exc...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exch...

WGU C001 BIOCHEMISTRY STUDY GUIDE QUESTIONS WITH VERIFIED ANSWERS Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chainto a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatog...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 Chapter 1: Overview of the Molecular Biology Geber 1800s: He developed ____ that make ways for molecular biology. From 1700s - 1900s there are huge advancements in ___ (start studying small molecules) 1930s- Understanding of ________ happen 1938s - "Molecular Biology" was written by ______. (First time the word molecular biology actually happen) 1944s - _____ shows DNA was the basis for genes 1953 - ____ and ____ determine...

Matching A) electrophoresis B) hydrophobic interaction C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) nucleases H) chromophore I) foaming J) high level expression K) 2-mercaptoethanol L) positive charge M) cation exchange N) pI O) chymotrypsin P) C-terminal amino acid Q Sodium dodecyl sulfate 1. One of the reasons the primary structure is important for a protein is that it determines the ______ the molecule adopts...

What is the proteome of the cell? correct answer: the full complex of proteins that can be produced using genetic material and post translational modifications What are some example of post translational modifications? correct answer: phosphorylation (signalling) GPI anchor (PM proteins) O-glycans (nuclear and cytosolic proteins) polyubiquitination (can induce protein degredation) How can you watch the purification of recombinant poly-His tagged GFP? correct answer: use Ni2+ immobili...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 What is recombinant DNA technology? - answerThe process of taking a gene from one source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and inserting that DNA into bacteria for further study/manipulation Probes - answerSmall pieces of DNA can be used to determine the presence or absence of an allele, which can indicate a predisposition to a specific disease Microarrays - answerAdvance probe-b...

QBM Exam 1 Questions And Answers With Complete Solutions 2024 Chapter 1: Overview of the Molecular Biology Geber 1800s: He developed ____ that make ways for molecular biology. From 1700s - 1900s there are huge advancements in ___ (start studying small molecules) 1930s- Understanding of ________ happen 1938s - "Molecular Biology" was written by ______. (First time the word molecular biology actually happen) 1944s - _____ shows DNA was the basis for genes 1953 - ____ and ____ determine...

QBM Final Exam Questions And Answers With Complete Solutions 2024 What is recombinant DNA technology? - answerThe process of taking a gene from one source (like human DNA) and joining it with another piece of DNA (like bacterial plasmid) and inserting that DNA into bacteria for further study/manipulation Probes - answerSmall pieces of DNA can be used to determine the presence or absence of an allele, which can indicate a predisposition to a specific disease Microarrays - answerAdvance pro...